The CBDC has been connected with the United Payments Interface (UPI), an immediate digital platform, by Indian private lender HDFC Bank.
According to media reports, it is the first Indian bank to provide this level of interoperability.
The action comes just days after RBI deputy governor T Rabi Sankar stated that such interoperability would be adopted in July.
Over 100,000 clients and 170,000 merchants have been successfully enrolled in pilot programmes utilising the central bank digital currency (CBDC) by HDFC Bank.
The e-rupee programme is said to be looking into the possible benefits of a digital substitute to real cash.
Through a single Quick Response code, businesses and customers would be able to make payments in e-rupee and UPI.
Under RBI oversight, the country intends to use blockchain distributed-ledger technology.